Abstract
Silica nanoparticles as a nonviral vector for in vivo gene therapy neither surface functionalized SiNp1 is neither "a cationic ion" nor a surface (encapsulation) nor SiNp2 (adsorption). p53 gene expression in the breast upon (i.v) administration. SiNp1 showed a 50- and 100-fold transfection activity, tumor growth inhibition, animal survival (80%), and high levels of p53 and Bax were detected in the sera of treated animals compared to SiNp2 or naked pCMV/p53, respectively. These results demonstrate for improvements in the both systems. This study suggests that nonviral vector systems will have important roles in achieving the impermanent gene transfer in vivo.
Keywords:
Breast cancer; gene delivery; in vivo transfection; pCMV/p53; silica nanoparticles.
MeSH terms
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Animals
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Breast Neoplasms / genetics
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Breast Neoplasms / mortality
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Breast Neoplasms / pathology
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Breast Neoplasms / therapy*
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Female
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Gene Expression Regulation, Neoplastic*
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Gene Transfer Techniques*
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Genetic Therapy / methods*
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Genetic Vectors / chemistry*
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Genetic Vectors / metabolism
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Humans
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Injections, Intralesional
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MCF-7 Cells
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Mice
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Mice, Inbred BALB C
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Micelles
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Nanoparticles / chemistry
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Nanoparticles / metabolism
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Neoplasm Transplantation
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Plasmids / chemistry
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Plasmids / metabolism
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Propylamines / chemistry
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Silanes / chemistry
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Silicon Dioxide / chemistry*
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Silicon Dioxide / metabolism
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Survival Analysis
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Tumor Burden
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Tumor Suppressor Protein p53 / agonists
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Tumor Suppressor Protein p53 / blood
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Tumor Suppressor Protein p53 / genetics
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bcl-2-Associated X Protein / agonists
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bcl-2-Associated X Protein / blood
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bcl-2-Associated X Protein / genetics
Substances
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BAX protein, human
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Micelles
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Propylamines
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Silanes
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Tumor Suppressor Protein p53
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bcl-2-Associated X Protein
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Silicon Dioxide
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vinyltriethoxysilane
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amino-propyl-triethoxysilane