Ratiometric detection and imaging of endogenous hypochlorite in live cells and in vivo achieved by using an aggregation induced emission (AIE)-based nanoprobe

Yong Huang; Peisheng Zhang; Meng Gao; Fang Zeng; Anjun Qin; Shuizhu Wu; Ben Zhong Tang

doi:10.1039/c6cc03415b

Ratiometric detection and imaging of endogenous hypochlorite in live cells and in vivo achieved by using an aggregation induced emission (AIE)-based nanoprobe

Chem Commun (Camb). 2016 Jun 7;52(45):7288-91. doi: 10.1039/c6cc03415b. Epub 2016 May 16.

Authors

Yong Huang¹, Peisheng Zhang, Meng Gao, Fang Zeng, Anjun Qin, Shuizhu Wu, Ben Zhong Tang

Affiliation

¹ College of Materials Science & Engineering, State Key Lab of Luminescent Materials & Devices, South China University of Technology, Guangzhou 510640, P. R. China. shzhwu@scut.edu.cn.

PMID: 27180866
DOI: 10.1039/c6cc03415b

Abstract

An AIE-based fluorescent nanoprobe (MTPE-M) has been developed and used for ratiometric detection of hypochlorite with high selectivity and sensitivity. More importantly, its application in live cells and zebrafish for ratiometric imaging of endogenous ClO(-) has also been achieved.

MeSH terms

Animals
Fluorescent Dyes / chemistry*
Hydroxyl Radical / chemistry
Hypochlorous Acid / analysis*
Hypochlorous Acid / chemistry
Larva / chemistry
Larva / metabolism
Mice
Microscopy, Fluorescence
Nanoparticles / chemistry*
Optical Imaging
RAW 264.7 Cells
Spectrometry, Fluorescence
Spectrometry, Mass, Electrospray Ionization
Zebrafish / growth & development
Zebrafish / metabolism

Substances

Fluorescent Dyes
Hydroxyl Radical
Hypochlorous Acid