Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization.
Keywords: Ebola virus; RNA virus; Venezuelan equine encephalitis virus; Zika virus; fieldable platform; genomics; nanopore sequencing; sequencing; viruses.