Virus-induced gene silencing reveals signal transduction components required for the Pvr9-mediated hypersensitive response in Nicotiana benthamiana

Phu-Tri Tran; Hoseong Choi; Doil Choi; Kook-Hyung Kim

doi:10.1016/j.virol.2016.05.011

Virus-induced gene silencing reveals signal transduction components required for the Pvr9-mediated hypersensitive response in Nicotiana benthamiana

Virology. 2016 Aug:495:167-72. doi: 10.1016/j.virol.2016.05.011. Epub 2016 May 26.

Authors

Phu-Tri Tran¹, Hoseong Choi¹, Doil Choi², Kook-Hyung Kim³

Affiliations

¹ Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
² Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
³ Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea. Electronic address: kookkim@snu.ac.kr.

PMID: 27236305
DOI: 10.1016/j.virol.2016.05.011

Abstract

Resistance to pathogens mediated by plant resistance (R) proteins requires different signaling transduction components and pathways. Our previous studies revealed that a potyvirus resistance gene in pepper, Pvr9, confers a hypersensitive response (HR) to pepper mottle virus in Nicotiana benthamiana. Our results show that the Pvr9-mediated HR against pepper mottle virus infection requires HSP90, SGT1, NDR1, but not EDS1. These results suggest that the Pvr9-mediated HR is possibly related to the SA pathway but not the ET, JA, ROS or NO pathways.

Keywords: Hypersensitive response; Pvr9; Signal transduction; VIGS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Disease Resistance / genetics*
Disease Resistance / immunology
Gene Silencing*
Host-Pathogen Interactions / genetics*
Host-Pathogen Interactions / immunology
Nicotiana / virology*
Phenotype
Plant Diseases / genetics*
Plant Diseases / immunology
Plant Diseases / virology*
Plant Proteins / genetics*
Plant Viruses / physiology
Signal Transduction*

Substances

Plant Proteins