Assessment of the anti-metastatic properties of sanguiin H-6 in HUVECs and MDA-MB-231 human breast cancer cells

Bioorg Med Chem Lett. 2016 Jul 15;26(14):3291-3294. doi: 10.1016/j.bmcl.2016.05.050. Epub 2016 May 18.

Abstract

The anti-metastatic properties of sanguiin H-6 were examined in human umbilical vein vascular endothelial cells (HUVECs) and MDA-MB-231 human breast cancer cells. In HUVECs, sanguiin H-6 inhibited the density of migrated cells compared to that observed after treatment with the vehicle. In addition, sanguiin H-6 at a concentration of 6.25μM significantly blocked tube formation. Treatment with up to 25μM sanguiin H-6 had no effect on MDA-MB-231 cells, whereas treatment with 200μM sanguiin H-6 decreased cell viability. Sanguiin H-6 significantly decreased the expression levels of vascular endothelial growth factor (VEGF), phosphorylated Akt, and extracellular signal-regulated kinase 1/2 (ERK1/2) in MDA-MB-231 cells. These findings suggest that sanguiin H-6 is potentially useful as an anti-metastatic agent.

Keywords: Breast cancer; HUVEC; Metastasis; Sanguiin H-6; VEGF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology*
  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Female
  • Human Umbilical Vein Endothelial Cells / drug effects*
  • Humans
  • Hydrolyzable Tannins / chemical synthesis
  • Hydrolyzable Tannins / chemistry
  • Hydrolyzable Tannins / pharmacology*
  • Molecular Structure
  • Neoplasm Metastasis / drug therapy
  • Neoplasm Metastasis / pathology
  • Structure-Activity Relationship

Substances

  • Antineoplastic Agents
  • Hydrolyzable Tannins
  • sanguiin H 6