Extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp

Carbohydr Polym. 2016 Oct 20:151:348-357. doi: 10.1016/j.carbpol.2016.05.068. Epub 2016 May 20.

Abstract

In this research, extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp were investigated. A response surface methodology (RSM) with a Box-Behnken design (BBD) was used to optimize the extraction process. The maximum EAP yield was 9.82±0.38%, which is in good agreement with the predicted value (9.93±0.24%). Two homogeneous polysaccharides, EAP-1a and EAP-1b with molecular weights of 8.70kDa and 4.39kDa respectively, were prepared by DEAE-52 cellulose and Sephadex G-100 columns and characterized by HPLC, HPGPC, and FT-IR. Three polysaccharides (EAP, EAP-1a and EAP-1b) could stimulate macrophages to release NO and enhance phagocytic activities of RAW 264.7 cells in dose-dependent manner. Moreover, there was no significant difference between crude EAP group (400μg/mL) and positive control group (LPS) in effects on macrophages. The results implied that EAP had the potential to be developed as natural medicines or health foods.

Keywords: Characterization; Elaeagnus angustifolia L. pulp; Immunological activities; Optimized extraction; Polysaccharides.

MeSH terms

  • Animals
  • Chromatography / methods*
  • Dextrans
  • Elaeagnaceae / chemistry*
  • Fruit / chemistry
  • Macrophages / drug effects*
  • Mice
  • Polysaccharides / immunology
  • Polysaccharides / isolation & purification*
  • Polysaccharides / pharmacology*
  • RAW 264.7 Cells

Substances

  • Dextrans
  • Polysaccharides
  • sephadex