Saponin-Based Nanoemulsification Improves the Antioxidant Properties of Vitamin A and E in AML-12 Cells

Int J Mol Sci. 2016 Aug 26;17(9):1406. doi: 10.3390/ijms17091406.

Abstract

Our work aimed to investigate the protective effects of saponin-based nanoemulsions of vitamin A and E against oxidative stress-induced cellular damage in AML-12 cells. Saponin nanoemulsions of vitamin A (SAN) and vitamin E (SEN) were prepared by high-pressure homogenization and characterized in terms of size, zeta potential, and polydispersity index. SEN and SAN protect AML-12 cells against oxidative stress-induced cellular damage more efficiently via scavenging reactive oxygen species (ROS), and reducing DNA damage, protein carbonylation, and lipid peroxidation. These results provide valuable information for the development of nanoemulsion-based delivery systems that would improve the antioxidant properties of vitamin A and E.

Keywords: antioxidant capacity; nanoemulsification; saponin; vitamin A; vitamin E.

MeSH terms

  • Antioxidants / chemistry*
  • Antioxidants / pharmacology*
  • Emulsions / chemistry*
  • Hepatocytes / drug effects
  • Hepatocytes / metabolism
  • Humans
  • Hydrogen Peroxide / pharmacology*
  • Nanostructures / chemistry*
  • Oxidative Stress / drug effects
  • Reactive Oxygen Species / metabolism
  • Saponins / chemistry*
  • Vitamin A / chemistry*
  • Vitamin A / pharmacology*
  • Vitamin E / chemistry*
  • Vitamin E / pharmacology*

Substances

  • Antioxidants
  • Emulsions
  • Reactive Oxygen Species
  • Saponins
  • Vitamin A
  • Vitamin E
  • Hydrogen Peroxide