Performance and microbial community of a sequencing batch biofilm reactor treating synthetic mariculture wastewater under long-term exposure to norfloxacin

Dong Zheng; Qingbo Chang; Zhiwei Li; Mengchun Gao; Zonglian She; Xuejiao Wang; Liang Guo; Yangguo Zhao; Chunji Jin; Feng Gao

doi:10.1016/j.biortech.2016.09.114

Performance and microbial community of a sequencing batch biofilm reactor treating synthetic mariculture wastewater under long-term exposure to norfloxacin

Bioresour Technol. 2016 Dec:222:139-147. doi: 10.1016/j.biortech.2016.09.114. Epub 2016 Sep 30.

Authors

Dong Zheng¹, Qingbo Chang², Zhiwei Li², Mengchun Gao³, Zonglian She², Xuejiao Wang⁴, Liang Guo², Yangguo Zhao², Chunji Jin², Feng Gao²

Affiliations

¹ Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Qingdao 266100, China.
² Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
³ Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. Electronic address: mengchungao@outlook.com.
⁴ College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.

PMID: 27716565
DOI: 10.1016/j.biortech.2016.09.114

Abstract

The performance and microbial community of a sequencing batch biofilm reactor (SBBR) treating synthetic mariculture wastewater were evaluated under long-term exposure to norfloxacin (NFX) due to the overuse of antibiotics during the mariculture. The COD and NH₄⁺-N removals had no distinct change at 0-6mgL^-1 NFX and were inhibited at 6-35mgL^-1 NFX. The specific oxygen uptake rate (SOUR), specific ammonium oxidation rate (SAOR), specific nitrite oxidation rate (SNOR) and specific nitrate reduction rate (SNRR) of the biofilm kept a decreasing tendency with the increase of NFX concentration from 0 to 35mgL^-1. The presence of NFX promoted the microorganisms to secrete more extracellular polymeric substances (EPS) and affected the chemical compositions of EPS. The microbial richness and diversity showed some obvious variations at different NFX concentrations. The present results demonstrated that NFX inhibited the SBBR performance and should decrease the NFX dosage in the mariculture.

Keywords: Mariculture; Microbial activity; Microbial diversity; Norfloxacin; SBBR.

MeSH terms

Aquaculture / methods*
Biofilms
Biological Oxygen Demand Analysis
Bioreactors / microbiology*
High-Throughput Nucleotide Sequencing
Microbial Consortia / drug effects*
Microbial Consortia / genetics
Nitrates / metabolism
Nitrification
Norfloxacin / pharmacology*
Waste Disposal, Fluid / instrumentation
Waste Disposal, Fluid / methods*
Wastewater

Substances

Nitrates
Waste Water
Norfloxacin