Characterization of glycerophospholipid molecular species in six species of edible clams by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry

Food Chem. 2017 Mar 15:219:419-427. doi: 10.1016/j.foodchem.2016.09.160. Epub 2016 Sep 28.

Abstract

The molecular species of glycerophosphocholine (GPCho), glycerophosphoethanolamine (GPEtn), glycerophosphoserine (GPSer), lysoglycerophosphocholine (LGPCho) and lysoglycerophosphoethanolamine (LGPEtn) from six species of edible clams were characterized by using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. At least 435, 453, 468, 443, 427 and 444 glycerophospholipid (GP) molecular species were characterized, respectively, from Cyclina sinensis, Mactra chinensis Philippi, Mactra veneriformis Reeve, Meretrix meretrix, Ruditapes philippinarum and Saxidomus purpurata. Most of the predominant GP molecular species in clam contained polyunsaturated fatty acids (PUFA), mainly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), indicating that clam is a potential resource of GP enriched PUFA. According to the amount of the major molecular species containing EPA and DHA, Cyclina sinensis was the best fit species for GPCho, Mactra veneriformis Reeve was the best fit species for GPEtn, Mactra chinensis Philippi was the best fit species for GPSer and LGPEtn, and Saxidomus purpurata was the best fit species for LGPCho.

Keywords: Clam; Glycerophospholipid; HPLC-ESI-MS/MS; Lysoglycerophospholipid; Molecular species.

MeSH terms

  • Animals
  • Bivalvia / chemistry*
  • Chromatography, High Pressure Liquid / methods*
  • Docosahexaenoic Acids / analysis*
  • Eicosapentaenoic Acid / analysis*
  • Phosphatidylethanolamines / analysis
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Tandem Mass Spectrometry / methods*

Substances

  • Phosphatidylethanolamines
  • glycerophosphoethanolamine
  • Docosahexaenoic Acids
  • Eicosapentaenoic Acid