Extraction and encapsulation of prodigiosin in chitosan microspheres for targeted drug delivery

S O Dozie-Nwachukwu; Y Danyuo; J D Obayemi; O S Odusanya; K Malatesta; W O Soboyejo

doi:10.1016/j.msec.2016.09.078

Extraction and encapsulation of prodigiosin in chitosan microspheres for targeted drug delivery

Mater Sci Eng C Mater Biol Appl. 2017 Feb 1:71:268-278. doi: 10.1016/j.msec.2016.09.078. Epub 2016 Sep 30.

Authors

S O Dozie-Nwachukwu¹, Y Danyuo², J D Obayemi³, O S Odusanya¹, K Malatesta⁴, W O Soboyejo⁵

Affiliations

¹ Department of Materials Science and Engineering, African University of Science and Technology (AUST) Abuja, Federal Capital Territory, Nigeria; Biotechnology and Genetic Engineering Advanced Laboratory, Sheda Science and Technology Complex (SHESTCO), P.M.B 186, Garki, Abuja, Federal Capital Territory, Nigeria.
² Department of Materials Science and Engineering, African University of Science and Technology (AUST) Abuja, Federal Capital Territory, Nigeria; Department of Materials Science and Engineering, Kwara State University, Malete, Nigeria.
³ Department of Materials Science and Engineering, African University of Science and Technology (AUST) Abuja, Federal Capital Territory, Nigeria; Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, USA.
⁴ Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, USA.
⁵ Department of Materials Science and Engineering, African University of Science and Technology (AUST) Abuja, Federal Capital Territory, Nigeria; Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, USA; Princeton Institute of Science and Technology of Materials (PRISM), Bowen Hall, 70 Prospect Street, Princeton, NJ 08544, USA. Electronic address: soboyejo@princeton.edu.

PMID: 27987708
DOI: 10.1016/j.msec.2016.09.078

Abstract

The encapsulation of drugs in polymeric materials has brought opportunities to the targeted delivery of chemotherapeutic agents. These polymeric delivery systems are capable of maximizing the therapeutic activity, as well as reducing the side effects of anti-cancer agents. Prodigiosin, a secondary metabolite extracted from the bacteria, Serratia marcescens, exhibits anti-cancer properties. Prodigiosin-loaded chitosan microspheres were prepared via water-in-oil (w/o) emulsion technique, using glutaraldehyde as a cross-linker. The morphologies of the microspheres were studied using scanning electron microscopy. The average sizes of the microspheres were between 40μm and 60μm, while the percentage yields ranged from 42±2% to 55.5±3%. The resulting encapsulation efficiencies were between 66.7±3% and 90±4%. The in-vitro drug release from the microspheres was characterized by zeroth order, first order and Higuchi and Korsmeyer-Peppas models.

Keywords: Chitosan; Drug release; Encapsulation; Microspheres; Prodigiosin; Serratia marcescens.

MeSH terms

Antineoplastic Agents* / chemistry
Antineoplastic Agents* / isolation & purification
Antineoplastic Agents* / pharmacokinetics
Antineoplastic Agents* / pharmacology
Drug Delivery Systems / methods*
Microspheres*
Prodigiosin* / chemistry
Prodigiosin* / isolation & purification
Prodigiosin* / pharmacokinetics
Prodigiosin* / pharmacology
Serratia marcescens / chemistry*

Substances

Antineoplastic Agents
Prodigiosin