A Phage-Like IncY Plasmid Carrying the mcr-1 Gene in Escherichia coli from a Pig Farm in China

Chunping Zhang; Yuqing Feng; Fei Liu; Hui Jiang; Zhina Qu; Meng Lei; Jianfeng Wang; Bing Zhang; Yongfei Hu; Jiabo Ding; Baoli Zhu

doi:10.1128/AAC.02035-16

A Phage-Like IncY Plasmid Carrying the mcr-1 Gene in Escherichia coli from a Pig Farm in China

Antimicrob Agents Chemother. 2017 Feb 23;61(3):e02035-16. doi: 10.1128/AAC.02035-16. Print 2017 Mar.

Authors

Chunping Zhang¹, Yuqing Feng^{2

3

4}, Fei Liu^{2

5

3}, Hui Jiang¹, Zhina Qu⁶, Meng Lei⁷, Jianfeng Wang⁷, Bing Zhang⁷, Yongfei Hu^{2

5

3}, Jiabo Ding⁸, Baoli Zhu^{9

5

3}

Affiliations

¹ China Institute of Veterinary Drug Control, Beijing, China.
² CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
³ Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing, China.
⁴ University of Chinese Academy of Sciences, Beijing, China.
⁵ Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
⁶ China Animal Health and Epidemiology Center, Qingdao, China.
⁷ Core Genomic Facility of Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
⁸ China Institute of Veterinary Drug Control, Beijing, China dingjiabo@126.com zhubaoli@im.ac.cn.
⁹ CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China dingjiabo@126.com zhubaoli@im.ac.cn.

Abstract

We report here a new type of plasmid that carries the mcr-1 gene, the pMCR-1-P3 plasmid, harbored in an Escherichia coli strain isolated from a pig farm in China. pMCR-1-P3 belongs to the IncY incompatibility group and is a phage-like plasmid that contains a large portion of phage-related sequences. The backbone of this plasmid is different from that of other mcr-1-carrying plasmids reported previously.

Keywords: DNA sequencing; antibiotics; plasmid.

MeSH terms

Animal Husbandry
Animals
Bacteriophages / genetics
Bacteriophages / metabolism
Base Sequence
China
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli / metabolism
Escherichia coli Infections / microbiology
Escherichia coli Infections / veterinary*
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism
Gene Expression
Open Reading Frames
Plasmids / chemistry*
Plasmids / metabolism
Sequence Analysis, DNA
Swine
Swine Diseases / microbiology*

Substances

Escherichia coli Proteins
MCR-1 protein, E coli