Lactate Detection in Tumor Cell Cultures Using Organic Transistor Circuits

Adv Mater. 2017 Apr;29(13). doi: 10.1002/adma.201605744. Epub 2017 Jan 30.

Abstract

A biosensing platform based on an organic transistor circuit for metabolite detection in highly complex biological media is introduced. The sensor circuit provides inherent background subtraction allowing for highly specific, sensitive lactate detection in tumor cell cultures. The proposed sensing platform paves the way toward rapid, label-free, and cost-effective clinically relevant in vitro diagnostic tools.

Keywords: Warburg effect; Wheatstone bridge circuit; organic electrochemical transistor; poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate); tumor cell cultures.

MeSH terms

  • Animals
  • Biosensing Techniques / methods*
  • Calibration
  • Cattle
  • Cell Culture Techniques
  • Chitosan / chemistry
  • Electric Impedance
  • Electrochemical Techniques / methods*
  • Equipment Design
  • Ferrous Compounds / chemistry
  • Humans
  • Lactic Acid / analysis*
  • Leukocytes, Mononuclear / chemistry
  • Metallocenes / chemistry
  • Mixed Function Oxygenases / chemistry
  • Neoplasms / chemistry*
  • Neoplasms / diagnosis*
  • Polystyrenes
  • Sensitivity and Specificity
  • Serum Albumin, Bovine / chemistry
  • Thiophenes
  • Transistors, Electronic

Substances

  • Ferrous Compounds
  • Metallocenes
  • Polystyrenes
  • Thiophenes
  • poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate)
  • Serum Albumin, Bovine
  • Lactic Acid
  • Chitosan
  • Mixed Function Oxygenases
  • lactate 2-monooxygenase
  • ferrocene