Graphene oxide-SiO2 nanocomposite as the adsorbent for extraction and preconcentration of plant hormones for HPLC analysis

Xiaona Zhang; Jiahua Niu; Xiaoting Zhang; Rui Xiao; Minghua Lu; Zongwei Cai

doi:10.1016/j.jchromb.2017.01.004

Graphene oxide-SiO₂ nanocomposite as the adsorbent for extraction and preconcentration of plant hormones for HPLC analysis

J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Mar 1:1046:58-64. doi: 10.1016/j.jchromb.2017.01.004. Epub 2017 Jan 5.

Authors

Xiaona Zhang¹, Jiahua Niu¹, Xiaoting Zhang¹, Rui Xiao¹, Minghua Lu², Zongwei Cai³

Affiliations

¹ Institute of Environmental and Analysis Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China.
² Institute of Environmental and Analysis Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China. Electronic address: mhlu@henu.edu.cn.
³ State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, China. Electronic address: zwcai@hkbu.edu.hk.

PMID: 28135698
DOI: 10.1016/j.jchromb.2017.01.004

Abstract

In this research, a modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method based on graphene oxide@SiO₂(SiO₂@GO) nanocomposite as adsorbent of dispersive solid-phase extraction (dSPE) combined with high performance liquid chromatography (HPLC) for the analysis of four plant hormones in different plants was established. The as-prepared SiO₂@GO was characterized by scanning electron microscopy, transmission electron microscopy and infrared spectroscopy. The experimental conditions for dSPE, including the ratio of material to liquid, pH of sample, adsorption and desorption time, desorption temperature as well as desorption solution, were investigated. The detection limits for the analysis of indole-3-acetic acid, indole-3-butyric acid, 1-naphthylacetic acid and abscisic acid were achieved below 0.05μgmL^-1. The established method was applied to the analysis of the plant hormones in fruits, vegetables and other food samples. The obtained results indicated that the method was sensitive, accurate, convenient and quick, which provided an alternative analytical approach for plant hormones in complex matrices.

Keywords: Dispersive solid-phase extraction; Graphene oxide-SiO(2) nanocomposite; HPLC; Plant hormones; QuEChERS.

MeSH terms

Chromatography, High Pressure Liquid / methods*
Graphite / chemistry*
Limit of Detection
Linear Models
Plant Growth Regulators / analysis*
Plant Growth Regulators / chemistry
Plant Growth Regulators / isolation & purification*
Reproducibility of Results
Silicon Dioxide / chemistry*
Solid Phase Extraction / methods*

Substances

Plant Growth Regulators
Silicon Dioxide
Graphite