Bioinspired Polydopamine-Coated Hemoglobin as Potential Oxygen Carrier with Antioxidant Properties

Quan Wang; Ruirui Zhang; Mingzi Lu; Guoxing You; Ying Wang; Gan Chen; Caiyan Zhao; Zhen Wang; Xiang Song; Yan Wu; Lian Zhao; Hong Zhou

doi:10.1021/acs.biomac.7b00077

Bioinspired Polydopamine-Coated Hemoglobin as Potential Oxygen Carrier with Antioxidant Properties

Biomacromolecules. 2017 Apr 10;18(4):1333-1341. doi: 10.1021/acs.biomac.7b00077. Epub 2017 Mar 28.

Authors

Quan Wang¹, Ruirui Zhang^{2

3}, Mingzi Lu¹, Guoxing You¹, Ying Wang¹, Gan Chen¹, Caiyan Zhao², Zhen Wang¹, Xiang Song¹, Yan Wu², Lian Zhao¹, Hong Zhou¹

Affiliations

¹ Beijing Institute of Transfusion Medicine, Beijing Key Laboratory of Blood Safety and Supply Technologies, 100039 Beijing, People's Republic of China.
² National Center for Nanoscience and Technology , 100190 Beijing, People's Republic of China.
³ Beijing Key Laboratory of Ionic Liquids Clean Process, Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences , 100190 Beijing, People's Republic of China.

PMID: 28323418
DOI: 10.1021/acs.biomac.7b00077

Abstract

Oxidative side reaction is one of the major factors hindering the development of hemoglobin-based oxygen carriers (HBOCs). To avoid the oxidative toxicity, we designed and synthesized polydopamine-coated hemoglobin (Hb-PDA) nanoparticles via simple one-step assemblage without any toxic reagent. Hb-PDA nanoparticles showed oxidative protection of Hb by inhibiting the generation of methemoglobin (MetHb) and ferryl (Fe IV) Hb, as well as excellent antioxidant properties by scavenging free radicals and reactive oxygen species (ROS). Interestingly, the scavenging rate of Hb-PDA nanoparticles for ABTS⁺ radical is at most 89%, while for DPPH radical it reaches 49%. In addition, Hb-PDA efficiently reduced the intracellular H₂O₂-induced ROS generation. Moreover, Hb-PDA nanoparticles exhibited high oxygen affinity, low effect on blood constituents, and low cytotoxicity. The results indicate that polydopamine-coated hemoglobin might be a promising approach for constructing novel oxygen carriers with the capacity to reduce oxidative side reaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antioxidants / administration & dosage
Antioxidants / adverse effects
Antioxidants / chemistry
Antioxidants / pharmacology*
Biocompatible Materials / administration & dosage
Biocompatible Materials / adverse effects
Biocompatible Materials / chemistry
Biocompatible Materials / pharmacology*
Biphenyl Compounds / chemistry
Blood Substitutes*
Cattle
Cell Survival / drug effects
Cells, Cultured
Cross-Linking Reagents / chemistry
Erythrocytes / drug effects
Erythrocytes / metabolism
Hemoglobins / administration & dosage
Hemoglobins / adverse effects
Hemoglobins / chemistry
Hemoglobins / pharmacology*
Hemolysis / drug effects
Human Umbilical Vein Endothelial Cells
Indoles / administration & dosage
Indoles / adverse effects
Indoles / chemistry
Indoles / pharmacology*
Male
Myocytes, Cardiac / drug effects
Myocytes, Cardiac / metabolism
Nanoparticles / chemistry
Oxygen / chemistry*
Picrates / chemistry
Platelet Aggregation / drug effects
Polymers / administration & dosage
Polymers / adverse effects
Polymers / chemistry
Polymers / pharmacology*
Rats, Wistar
Reactive Oxygen Species / metabolism
Spectroscopy, Fourier Transform Infrared

Substances

Antioxidants
Biocompatible Materials
Biphenyl Compounds
Blood Substitutes
Cross-Linking Reagents
Hemoglobins
Indoles
Picrates
Polymers
Reactive Oxygen Species
polydopamine
1,1-diphenyl-2-picrylhydrazyl
Oxygen