Detection of Prohibited Fish Drugs Using Silver Nanowires as Substrate for Surface-Enhanced Raman Scattering

Jia Song; Yiqun Huang; Yuxia Fan; Zhihui Zhao; Wansong Yu; Barbara A Rasco; Keqiang Lai

doi:10.3390/nano6090175

Detection of Prohibited Fish Drugs Using Silver Nanowires as Substrate for Surface-Enhanced Raman Scattering

Nanomaterials (Basel). 2016 Sep 21;6(9):175. doi: 10.3390/nano6090175.

Authors

Jia Song¹, Yiqun Huang^{2

3}, Yuxia Fan^{4

5}, Zhihui Zhao^{6

7}, Wansong Yu⁸, Barbara A Rasco⁹, Keqiang Lai^{10

11}

Affiliations

¹ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. susanjia123@163.com.
² College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. yiqunh@hotmail.com.
³ School of Food Science, Washington State University, Pullman, WA 99165, USA. yiqunh@hotmail.com.
⁴ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. yxfan@shou.edu.cn.
⁵ Engineering Research Center of Food Thermal-processing Technology, Shanghai Ocean University, Shanghai 201306, China. yxfan@shou.edu.cn.
⁶ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. zhihuiz2015@sohu.com.
⁷ Engineering Research Center of Food Thermal-processing Technology, Shanghai Ocean University, Shanghai 201306, China. zhihuiz2015@sohu.com.
⁸ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. dasong1990@126.com.
⁹ School of Food Science, Washington State University, Pullman, WA 99165, USA. rasco@wsu.edu.
¹⁰ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. kqlai@shou.edu.cn.
¹¹ Engineering Research Center of Food Thermal-processing Technology, Shanghai Ocean University, Shanghai 201306, China. kqlai@shou.edu.cn.

Abstract

Surface-enhanced Raman scattering or surface-enhanced Raman spectroscopy (SERS) is a promising detection technology, and has captured increasing attention. Silver nanowires were synthesized using a rapid polyol method and optimized through adjustment of the molar ratio of poly(vinyl pyrrolidone) and silver nitrate in a glycerol system. Ultraviolet-visible spectrometry, X-ray diffraction, and transmission electron microscopy were used to characterize the silver nanowires. The optimal silver nanowires were used as a SERS substrate to detect prohibited fish drugs, including malachite green, crystal violet, furazolidone, and chloramphenicol. The SERS spectra of crystal violet could be clearly identified at concentrations as low as 0.01 ng/mL. The minimum detectable concentration for malachite green was 0.05 ng/mL, and for both furazolidone and chloramphenicol were 0.1 μg/mL. The results showed that the as-prepared Ag nanowires SERS substrate exhibits high sensitivity and activity.

Keywords: SERS; chloramphenicol; crystal violet; furazolidone; glycerol; malachite green; silver nanowires.