Polydatin down-regulates the phosphorylation level of Creb and induces apoptosis in human breast cancer cell

PLoS One. 2017 May 3;12(5):e0176501. doi: 10.1371/journal.pone.0176501. eCollection 2017.

Abstract

Polydatin (PD), a component isolated from Polygonum cuspidatum, has a number of biological functions. However, the antitumor activity of PD has been poorly investigated. In this study, the effect of PD on cell proliferation was evaluated by thiazolyl blue tetrazolium bromide assay. Cell cycle distribution and apoptosis were investigated by flow cytometry. The phosphorylation levels of panel of phosphor-kinases were detected by human phospho-kinase arrays. The expression of several proteins associated with cell cycle and apoptosis were analyzed by Western blot analysis. Results showed that PD effectively inhibited the growth of MDA-MB-231 and MCF-7 breast cancer cell lines. Cell cycle analysis demonstrated that PD induced S-phase cell cycle arrest. Human phosphor-kinase arrays showed that the phosphorylation level of cAMP response element-bingding proteins(Creb) was down-regulated, and the results were further confirmed by Western blot analysis. Western blot analysis showed that the expression of protein of cyclin D1 decreased in a time- and dose- dependent manner. Results suggest that PD is a potential therapeutic natural compound.

MeSH terms

  • Apoptosis / drug effects*
  • Breast Neoplasms / pathology*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cyclic AMP Response Element-Binding Protein / metabolism*
  • Down-Regulation / drug effects*
  • Glucosides / pharmacology*
  • Humans
  • Phosphorylation
  • S Phase / drug effects
  • Stilbenes / pharmacology*

Substances

  • CREB1 protein, human
  • Cyclic AMP Response Element-Binding Protein
  • Glucosides
  • Stilbenes
  • polydatin

Grants and funding

The current study was supported by grants from Suzhou Science and Technology Program (SYS201345), Bureau of Public Health of Jiangsu Province (H201413), pre-research project from the second affiliated hospital of Soochow university (SDFEYGJ1609) and the second affiliated hospital of Soochow university clinical discipline group project funding (XKQ 2015008). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.