IL-10-producing regulatory B cells (B10 cells), IL-17+ T cells and autoantibodies in systemic sclerosis

Clin Immunol. 2017 Nov:184:26-32. doi: 10.1016/j.clim.2017.04.013. Epub 2017 May 11.

Abstract

We aimed to analyze IL-10+ Breg (B10) cells, found to be reduced in systemic sclerosis (SSc), in relation to SSc-specific autoAbs and IL-17+ and IFNγ+ T cells in SSc. Peripheral blood B10 cells from 26 patients with SSc positive for anti-Topo I or anti-Cen autoAbs, and 12 healthy controls (HC) were studied by flow cytometry. IL-17+ and IFNγ+ T cells were also studied. B10 cells did not correlate with anti-Topo I or anti-Cen Ab levels but were inversely correlated with IL-17+ CD3+ cells and IFNγ+ CD3+ cells. IL-17+ CD3+ cells did not correlate with autoAb levels, but IFN-γ+ CD3+ cells were inversely correlated with anti-Topo I levels. In conclusion, in SSc, B10 cells did not correlate with SSc-specific autoAbs and exhibited an inverse correlation with IL-17+ T cells and IFNγ+ T cells.

Keywords: Autoantibody; Autoimmunity; B regulatory cells; Scleroderma; T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antibodies, Antinuclear / immunology
  • Autoantibodies / immunology*
  • B-Lymphocytes, Regulatory / immunology*
  • CD3 Complex / immunology
  • Case-Control Studies
  • DNA Topoisomerases, Type I / immunology
  • Female
  • Flow Cytometry
  • Humans
  • Interferon-gamma / immunology
  • Interleukin-10 / immunology
  • Interleukin-17 / immunology
  • Male
  • Middle Aged
  • Scleroderma, Systemic / immunology*
  • T-Lymphocytes / immunology*
  • Young Adult

Substances

  • Antibodies, Antinuclear
  • Autoantibodies
  • CD3 Complex
  • IFNG protein, human
  • IL10 protein, human
  • Interleukin-17
  • anticentromere antibody
  • Interleukin-10
  • Interferon-gamma
  • DNA Topoisomerases, Type I