Exploring the clonal evolution of CD133/aldehyde-dehydrogenase-1 (ALDH1)-positive cancer stem-like cells from primary to recurrent high-grade serous ovarian cancer (HGSOC). A study of the Ovarian Cancer Therapy-Innovative Models Prolong Survival (OCTIPS) Consortium

Eur J Cancer. 2017 Jul:79:214-225. doi: 10.1016/j.ejca.2017.04.016. Epub 2017 May 16.

Abstract

Background: High-grade serous ovarian cancer (HGSOC) causes 80% of all ovarian cancer (OC) deaths. In this setting, the role of cancer stem-like cells (CSCs) is still unclear. In particular, the evolution of CSC biomarkers from primary (pOC) to recurrent (rOC) HGSOCs is unknown. Aim of this study was to investigate changes in CD133 and aldehyde dehydrogenase-1 (ALDH1) CSC biomarker expression in pOC and rOC HGSOCs.

Methods: Two-hundred and twenty-four pOC and rOC intrapatient paired tissue samples derived from 112 HGSOC patients were evaluated for CD133 and ALDH1 expression using immunohistochemistry (IHC); pOCs and rOCs were compared for CD133 and/or ALDH1 levels. Expression profiles were also correlated with patients' clinicopathological and survival data.

Results: Some 49.1% of the patient population (55/112) and 37.5% (42/112) pOCs were CD133+ and ALDH1+ respectively. CD133+ and ALDH1+ samples were detected in 33.9% (38/112) and 36.6% (41/112) rOCs. CD133/ALDH1 coexpression was observed in 23.2% (26/112) and 15.2% (17/112) of pOCs and rOCs respectively. Pairwise analysis showed a significant shift of CD133 staining from higher (pOCs) to lower expression levels (rOCs) (p < 0.0001). Furthermore, all CD133 + pOC patients were International Federation of Gynaecology and Obstetrics (FIGO)-stage III/IV (p < 0.0001) and had significantly worse progression-free interval (PFI) (p = 0.04) and overall survival (OS) (p = 0.02). On multivariate analysis, CD133/ALDH1 coexpression in pOCs was identified as independent prognostic factor for PFI (HR: 1.64; 95% CI: 1.03-2.60; p = 0.036) and OS (HR: 1.71; 95% CI: 1.01-2.88; p = 0.045). Analysis on 52 pts patients with known somatic BRCA status revealed that BRCA mutations did not influence CSC biomarker expression.

Conclusions: The study showed that CD133/ALDH1 expression impacts HGSOC patients' survival and first suggests that CSCs might undergo phenotypic change during the disease course similarly to non stem-like cancer cells, providing also a first evidence that there is no correlation between CSCs and BRCA status.

Keywords: ALDH1; Aldehyde dehydrogenase-1; BRCA; CD133; Cancer stem-like cell; Ovarian cancer; Prognosis; Survival.

Publication types

  • Multicenter Study

MeSH terms

  • AC133 Antigen / metabolism*
  • Adult
  • Aged
  • Aldehyde Dehydrogenase 1 Family
  • Antineoplastic Agents / therapeutic use
  • BRCA2 Protein / metabolism
  • Biomarkers, Tumor / metabolism*
  • Carcinoma, Ovarian Epithelial
  • Clonal Evolution
  • Female
  • Humans
  • Isoenzymes / metabolism*
  • Middle Aged
  • Neoplasm Recurrence, Local / enzymology
  • Neoplasm Recurrence, Local / mortality
  • Neoplasm Recurrence, Local / pathology
  • Neoplasm, Residual / enzymology
  • Neoplasm, Residual / mortality
  • Neoplasm, Residual / pathology
  • Neoplasms, Glandular and Epithelial / enzymology*
  • Neoplasms, Glandular and Epithelial / mortality
  • Neoplasms, Glandular and Epithelial / pathology
  • Neoplastic Stem Cells / enzymology*
  • Neoplastic Stem Cells / pathology
  • Ovarian Neoplasms / enzymology*
  • Ovarian Neoplasms / mortality
  • Ovarian Neoplasms / pathology
  • Platinum Compounds / therapeutic use
  • Retinal Dehydrogenase / metabolism*
  • Survival Analysis
  • Ubiquitin-Protein Ligases / metabolism

Substances

  • AC133 Antigen
  • Antineoplastic Agents
  • BRCA2 Protein
  • BRCA2 protein, human
  • Biomarkers, Tumor
  • Isoenzymes
  • Platinum Compounds
  • Aldehyde Dehydrogenase 1 Family
  • ALDH1A1 protein, human
  • Retinal Dehydrogenase
  • BRAP protein, human
  • Ubiquitin-Protein Ligases