VARIATION IN RIBOSOMAL DNA AMONG BIOLOGICAL SPECIES OF ARMILLARIA, A GENUS OF ROOT-INFECTING FUNGI

James B Anderson; Steven S Bailey; Patricia J Pukkila

doi:10.1111/j.1558-5646.1989.tb02615.x

VARIATION IN RIBOSOMAL DNA AMONG BIOLOGICAL SPECIES OF ARMILLARIA, A GENUS OF ROOT-INFECTING FUNGI

Evolution. 1989 Dec;43(8):1652-1662. doi: 10.1111/j.1558-5646.1989.tb02615.x.

Authors

James B Anderson¹, Steven S Bailey¹, Patricia J Pukkila²

Affiliations

¹ Department of Botany, Erindale College, University of Toronto, Mississauga, Ontario, L5L 1C6, CANADA.
² Department of Biology, Curriculum in Genetics, University of North Carolina, Chapel Hill, NC, USA, 27599-3280.

PMID: 28564337
DOI: 10.1111/j.1558-5646.1989.tb02615.x

Abstract

Armillaria is a genus of root-infecting fungi composed of several biological species in North America and Europe. To examine relatedness among biological species, ribosomal DNA (rDNA) from one isolate was cloned and rDNAs from 30 isolates were mapped for eight restriction enzymes. The positions of the large (26S) and small (18S) rRNA cistrons were found by Northern hybridizations of total cellular RNA with rDNA subclones and by alignment of maps with conserved restriction sites present in rRNA genes of other fungi. Nine restriction-site and two length polymorphisms were observed. Eight North American (Roman numerals) and five European (species epithets) biological species could be placed in six classes with respect to rDNA maps (rDNA class 1: I and A. ostoyae; class 2: II; class 3: A. borealis; class 4: V, IX, and X; class 5: III, VII, A. lutea, and A. cepistipes; and class 6: VI and A. mellea). Most, but not all, polymorphisms were in intergenic regions.