Unravelling HIV-1 Latency, One Cell at a Time

Yik Lim Kok; Angela Ciuffi; Karin J Metzner

doi:10.1016/j.tim.2017.06.002

Unravelling HIV-1 Latency, One Cell at a Time

Trends Microbiol. 2017 Nov;25(11):932-941. doi: 10.1016/j.tim.2017.06.002. Epub 2017 Jun 28.

Authors

Yik Lim Kok¹, Angela Ciuffi², Karin J Metzner³

Affiliations

¹ Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Rämistrasse 100, 8091 Zurich, Switzerland; Institute of Medical Virology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
² Institute of Microbiology, University Hospital Center and University of Lausanne, Bugnon 48, CH-1011 Lausanne, Switzerland.
³ Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Rämistrasse 100, 8091 Zurich, Switzerland; Institute of Medical Virology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. Electronic address: karin.metzner@usz.ch.

PMID: 28668335
DOI: 10.1016/j.tim.2017.06.002

Abstract

A single virus is capable of infecting and replicating in a single cell. Recent advances across single-cell omics technologies - genomics, epigenomics, transcriptomics, epitranscriptomics, proteomics, and metabolomics - will offer unprecedented opportunities to gain more insights into the various aspects of the life cycle of viruses and their impact on the host cell. Here, using the human immunodeficiency virus type 1 (HIV-1) as an example, we summarize the current knowledge and the future potential of single-cell omics in the investigation of an important aspect of the life cycle of HIV-1 that represents a major hurdle in achieving viral eradication, HIV-1 latency.

Keywords: HIV-1 integration sites; human immunodeficiency virus type 1; single-cell omics; viral latency.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Genomics
HIV Infections / virology*
HIV-1 / growth & development*
Host-Pathogen Interactions
Humans
Metabolomics
Single-Cell Analysis / methods*
Virus Latency / physiology*