The continuing problem of poor transparency of reporting and use of inappropriate methods for RT-qPCR

Stephen Bustin

doi:10.1016/j.bdq.2017.05.001

The continuing problem of poor transparency of reporting and use of inappropriate methods for RT-qPCR

Biomol Detect Quantif. 2017 May 23:12:7-9. doi: 10.1016/j.bdq.2017.05.001. eCollection 2017 Jun.

Author

Stephen Bustin¹

Affiliation

¹ Faculty of Medical Science, Anglia Ruskin University, Bishop Hall Lane, Chelmsford, CM1 1SQ, UK.

Abstract

Attendance at this year's European Calcified Tissue Society's (ECTS) Congress reveals that the methods used to obtain qPCR results continue to be significantly flawed and that and their reporting remain inadequate.

Keywords: Bone; Expression profiling; RNA; Reverse transcription; qPCR.