Ethylenediaminetetraacetic acid (EDTA)-treated dentin matrix (TDM) is an enriched source of bioactive molecules. Therefore, it was hypothesized that fabrication of autogenous TDM on root dentinal walls of necrotic immature permanent teeth may allow more predictable outcome of revascularization treatments. Two young patients with permanent nonvital immature teeth were chosen for revascularization treatment. After appropriate disinfection of root canal system, TDM was fabricated on root dentinal walls using different dilutions of EDTA. Then, bleeding was induced in canals and calcium-enriched mixture (CEM) cement was placed over the blood clots. In all follow-up periods, both cases were asymptomatic and radiographic findings have shown a continued root development. Revascularization is a valuable treatment for nonvital immature teeth, allows continuation of root development. Modification of root regeneration through a TDM protocol may seem more predictable treatment and improve maturogenesis than traditional therapy.
Keywords: Calcium-enriched mixture; dentin-pulp regeneration; open apex; revascularization; treated dentin matrix.