Vacuolar degradation of chloroplast components: autophagy and beyond

Chloroplast degradation during natural or stress-induced senescence requires the participation of both plastidic and extraplastidic degradative pathways. As part of the extraplastidic pathways, chloroplasts export stroma, envelope, and thylakoid proteins in membrane-bound organelles that are ultimately degraded in vacuoles. Some of these pathways, such as the formation of senescence-associated vacuoles (SAVs) and CV-containing vesicles (CCVs), do not depend on autophagy, whereas delivery of Rubisco-containing bodies (RCBs), ATI1-PS (ATG8-interacting Protein 1) bodies, and small starch-like granule (SSLG) bodies is autophagy dependent. In addition, autophagy of entire chloroplasts delivers damaged chloroplasts into the vacuolar lumen for degradation. This review summarizes the autophagy-dependent and independent trafficking mechanisms by which plant cells degrade chloroplast components in vacuoles.