The inflammatory reaction around the implant after implant placement is important not only for osseointegration but also for long-term implant survivals. In our study, GL13K, an antimicrobial peptide, was immobilized onto titanium surfaces to improve its anti-inflammatory properties. The method of silanization was used to immobilize the GL13K, which was confirmed by X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy, water contact angle measurement. DAPI fluorescence staining and Cell Counting Kit-8 (CCK-8) were used to measure the cell attachment and cell viability of the RAW264.7, which indicated a good cytocompatibility. Cellular morphology of RAW264.7 on modified surfaces showed less cell pseudopod. ELISA and qRT-PCR were performed to measure the inflammatory activity of the modified titanium surfaces. The secretion levels of pro-inflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-alpha (TNF-α) and inducible nitric oxide synthase (iNOS) were downregulated at 12h, 24h, and 48h, while the anti-inflammatory cytokines IL-10 and arginase were upregulated at 12h, 24h, and 48h. All results indicate that the GL13K-coated titanium surfaces make the inflammatory process towards a less pro-inflammatory, which may promote the process of osseointegration.
Keywords: Antimicrobial peptide; Inflammatory; Macrophage; Silanization; Titanium.
Copyright © 2017. Published by Elsevier B.V.