blaNDM-1-producing multidrug-resistant Escherichia coli isolated from a companion dog in China

Lanqing Cui; Lei Lei; Yuan Lv; Rongmin Zhang; Xiaoyu Liu; Mei Li; Fan Zhang; Yang Wang

doi:10.1016/j.jgar.2017.10.021

bla_NDM-1-producing multidrug-resistant Escherichia coli isolated from a companion dog in China

J Glob Antimicrob Resist. 2018 Jun:13:24-27. doi: 10.1016/j.jgar.2017.10.021. Epub 2017 Nov 2.

Authors

Lanqing Cui¹, Lei Lei², Yuan Lv³, Rongmin Zhang², Xiaoyu Liu², Mei Li², Fan Zhang¹, Yang Wang⁴

Affiliations

¹ Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, China.
² Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
³ Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, China. Electronic address: lvyuan0901@sina.com.
⁴ Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China. Electronic address: wangyang@cau.edu.cn.

PMID: 29102775
DOI: 10.1016/j.jgar.2017.10.021

Abstract

Objectives: This study characterised a bla_NDM-1-producing Escherichia coli isolate from a companion dog.

Methods: The E. coli strain was isolated from a surveillance study of carbapenem-resistant Gram-negative bacteria from companion animals in the Animal Teaching Hospital of China Agricultural University (Beijing, China) in 2013. Species identification was performed using an API 20E system and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Minimum inhibitory concentrations (MICs) of various antimicrobial agents were determined by agar dilution. Multilocus sequence typing (MLST) was performed and various carbapenemase genes, including bla_NDM, bla_IMP, bla_VIM, bla_SME, bla_IMI, bla_KPC, bla_GES, bla_SPM, bla_GIM, bla_NMC, bla_DIM, bla_SIM, bla_OXA-23, bla_OXA-24, bla_OXA-48, bla_OXA-51 and bla_OXA-58 were screened by PCR. S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and Southern blotting as well as a modified random primer walking strategy were performed to analyse the location and genetic environment of bla_NDM-1.

Results: An E. coli isolate belonging to ST167 was found to be positive for the bla_NDM-1 gene and exhibited resistance to β-lactams, tetracycline, gentamicin, fosfomycin and ciprofloxacin. The bla_NDM-1 gene in this strain was located on an ca. 150-kb plasmid and the flanking sequences of the bla_NDM-1-carrying region (a common gene cluster, ΔISAba125-bla_NDM-1-ble-trpF-ΔdsbC) exhibited >99% identity to the corresponding regions of bla_CTX-M-15-harboring plasmids in nosocomial E. coli ST131 isolates.

Conclusions: This is the first report of bla_NDM-1-producing ST167 E. coli in a companion dog. Companion animals harbouring carbapenemase-producing isolates are an upcoming public health threat and it is worthy paying attention to the emergence of carbapenem resistance in companion animals.

Keywords: Carbapenem resistance; Carbapenemases; Companion animal; Enterobacteriaceae; ST167.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Bacterial Typing Techniques
China
Dog Diseases / microbiology*
Dogs
Drug Resistance, Multiple, Bacterial*
Electrophoresis, Gel, Pulsed-Field
Escherichia coli / drug effects*
Escherichia coli / enzymology
Escherichia coli / genetics
Escherichia coli / isolation & purification*
Escherichia coli Infections / microbiology
Escherichia coli Infections / veterinary*
Male
Microbial Sensitivity Tests
Multilocus Sequence Typing
Pets
Plasmids / analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
beta-Lactamases / metabolism*

Substances

Anti-Bacterial Agents
beta-Lactamases
beta-lactamase NDM-1