In vivo selection of a multidrug-resistant Aeromonas salmonicida during medicinal leech therapy

E Ruppé; A Cherkaoui; N Wagner; G C La Scala; J-Y Beaulieu; M Girard; J Frey; V Lazarevic; J Schrenzel

doi:10.1016/j.nmni.2017.10.005

In vivo selection of a multidrug-resistant Aeromonas salmonicida during medicinal leech therapy

New Microbes New Infect. 2017 Oct 10:21:23-27. doi: 10.1016/j.nmni.2017.10.005. eCollection 2018 Jan.

Authors

E Ruppé¹, A Cherkaoui², N Wagner³, G C La Scala⁴, J-Y Beaulieu⁵, M Girard¹, J Frey⁶, V Lazarevic¹, J Schrenzel^{1

2}

Affiliations

¹ Genomic Research Laboratory, Division of Infectious Diseases, Switzerland.
² Bacteriology Laboratory, Division of Laboratory Medicine, Department of Genetics and Laboratory Medicine, Switzerland.
³ Pediatric Infectious Diseases, Department of Pediatrics, Switzerland.
⁴ Division of Pediatric Surgery, Department of Pediatrics, Switzerland.
⁵ Hand Surgery Unit, Division of Orthopedic Surgery, Department of Surgery, Geneva University Hospitals, Geneva, Switzerland.
⁶ Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland.

Abstract

We report the selection in a 15-year-old boy of a multidrug-resistant, extended-spectrum β-lactamase (ESBL)-producing Aeromonas salmonicida after medicinal leech therapy that required an antibiotic prophylaxis based on piperacillin/tazobactam and cotrimoxazole. Whole genome sequencing of the strain indeed revealed 13 antibiotic resistance genes, including the ESBL CTX-M-3 and the unusual β-lactamase SCO-1.

Keywords: Aeromonas salmonicida; medicinal leech therapy; multidrug resistance; whole genome sequencing.