Abstract
Cruciform DNA, a non-double helix form of DNA, can be generated as an intermediate in genetic recombination as well as from palindromic sequences under the effect of supercoiling. Eukaryotic cells are equipped with a DNA-binding protein that selectively recognizes cruciform DNA. Biochemical and immunological data showed that this protein is HMG1, an evolutionarily conserved, essential, and abundant component of the nucleus. The interaction with a ubiquitous protein points to a critical role for cruciform DNA conformations.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Base Sequence
-
Cloning, Molecular
-
DNA / genetics
-
DNA / metabolism*
-
Electrophoresis, Polyacrylamide Gel
-
High Mobility Group Proteins / genetics
-
High Mobility Group Proteins / isolation & purification
-
High Mobility Group Proteins / metabolism*
-
Immunoassay
-
Immunoblotting
-
Liver / analysis
-
Molecular Sequence Data
-
Molecular Weight
-
Nucleic Acid Conformation*
-
Peptide Fragments / genetics
-
Peptide Fragments / isolation & purification
-
Protein Biosynthesis
-
Rats
-
Transcription, Genetic
Substances
-
High Mobility Group Proteins
-
Peptide Fragments
-
DNA