The association of ribosomal protein L18 (RPL18) with infectious bursal disease virus viral protein VP3 enhances viral replication

Bin Wang; Xueyan Duan; Mengjiao Fu; Yanan Liu; Yongqiang Wang; Xiaoqi Li; Hong Cao; Shijun J Zheng

doi:10.1016/j.virusres.2017.12.009

The association of ribosomal protein L18 (RPL18) with infectious bursal disease virus viral protein VP3 enhances viral replication

Virus Res. 2018 Feb 2:245:69-79. doi: 10.1016/j.virusres.2017.12.009. Epub 2017 Dec 19.

Authors

Bin Wang¹, Xueyan Duan¹, Mengjiao Fu¹, Yanan Liu¹, Yongqiang Wang¹, Xiaoqi Li¹, Hong Cao¹, Shijun J Zheng²

Affiliations

¹ State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
² State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: sjzheng@cau.edu.cn.

PMID: 29273342
DOI: 10.1016/j.virusres.2017.12.009

Abstract

Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive avian disease caused by IBD virus (IBDV). IBDV VP3 is a multifunctional protein playing a key role in virus assembly and pathogenesis. To investigate the role of VP3 in pathogenesis, we transfected DF-1 cells with pRK5-FLAG-vp3 and found that VP3 enhanced type I interferon expression and suppressed IBDV replication. Furthermore we found that VP3 interacted with chicken Ribosomal Protein L18 (chRPL18) in host cells and knockdown of chRPL18 by RNAi significantly promoted Type I interferon expression and inhibited IBDV replication. Moreover, our data show that chicken double-stranded RNA-activated protein kinase (chPKR) interacted with both VP3 and chRPL18. Thus chRPL18 in association with VP3 and chPKR affects viral replication.

Keywords: IBDV VP3; IBDV replication; chPKR; chRPL18.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Chickens
Fibroblasts / immunology
Fibroblasts / virology*
Gene Expression
Gene Expression Regulation
Host-Pathogen Interactions
Infectious bursal disease virus / genetics*
Infectious bursal disease virus / growth & development
Infectious bursal disease virus / immunology
Interferon Type I / genetics*
Interferon Type I / immunology
Plasmids / chemistry
Plasmids / metabolism
Ribosomal Proteins / genetics*
Ribosomal Proteins / immunology
Signal Transduction
Transfection
Viral Structural Proteins / genetics*
Viral Structural Proteins / immunology
Virus Replication
eIF-2 Kinase / genetics*
eIF-2 Kinase / immunology

Substances

Interferon Type I
Ribosomal Proteins
VP3 protein, infectious bursal disease virus
Viral Structural Proteins
ribosomal protein L18
eIF-2 Kinase