Gp2.5, the multifunctional bacteriophage T7 single-stranded DNA binding protein

Alfredo J Hernandez; Charles C Richardson

doi:10.1016/j.semcdb.2018.03.018

Gp2.5, the multifunctional bacteriophage T7 single-stranded DNA binding protein

Semin Cell Dev Biol. 2019 Feb:86:92-101. doi: 10.1016/j.semcdb.2018.03.018. Epub 2018 Mar 28.

Authors

Alfredo J Hernandez¹, Charles C Richardson²

Affiliations

¹ Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
² Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA. Electronic address: ccr@hms.harvard.edu.

Abstract

The essential bacteriophage T7-encoded single-stranded DNA binding protein is the nexus of T7 DNA metabolism. Multiple layers of macromolecular interactions mediate its function in replication, recombination, repair, and the maturation of viral genomes. In addition to binding ssDNA, the protein binds to DNA polymerase and DNA helicase, regulating their activities. The protein displays potent homologous DNA annealing activity, underscoring its role in recombination.

Keywords: Bacteriophage T7; DNA replication annealing; Homologous; Recombination; Single-stranded DNA binding protein.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

MeSH terms

Bacteriophage T7 / chemistry*
Bacteriophage T7 / genetics
DNA Replication
DNA, Viral / chemistry
DNA, Viral / genetics
DNA, Viral / metabolism
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*

Substances

DNA, Viral
DNA-Binding Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding