Abstract
Five isocoumarin derivatives including three new compounds, aspergisocoumrins A-C (1-3), together with two known analogues, 8-dihydroxyisocoumarin-3-carboxylic acid (4) and dichlorodiaportin (5) were obtained from the culture of the endophytic fungus Aspergillus sp. HN15-5D derived from the fresh leaves of the mangrove plant Acanthus ilicifolius. Their structures were elucidated using comprehensive spectroscopic methods. The double bond geometry of compounds 1 and 2 were assigned as E and Z on the basis of the distinct coupling constants, respectively. Compounds 1 and 2 showed cytotoxicity against MDA-MB-435 with IC50 values of 5.08 ± 0.88 and 4.98 ± 0.74 μM, respectively.
Keywords:
Aspergillus sp.; Cytotoxicity; Isocoumarins; Mangrove endophytic fungus.
MeSH terms
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification
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Anti-Bacterial Agents / pharmacology*
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / pharmacology*
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Aspergillus / chemistry*
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Bacillus subtilis / drug effects
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Dose-Response Relationship, Drug
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Drug Screening Assays, Antitumor
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Escherichia coli / drug effects
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Humans
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Isocoumarins / chemistry
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Isocoumarins / isolation & purification
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Isocoumarins / pharmacology*
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Klebsiella pneumoniae / drug effects
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Microbial Sensitivity Tests
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Molecular Structure
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Staphylococcus aureus / drug effects
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Staphylococcus epidermidis / drug effects
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Structure-Activity Relationship
Substances
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Anti-Bacterial Agents
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Antineoplastic Agents
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Isocoumarins