The long noncoding RNA lncPARP1 contributes to progression of hepatocellular carcinoma through up-regulation of PARP1

Heqiang Qi; Yuyan Lu; Jie Lv; Huita Wu; Jing Lu; Changmao Zhang; Sheng Zhang; Qing Bao; Xiuming Zhang; Chengrong Xie; Zhenyu Yin

doi:10.1042/BSR20180703

The long noncoding RNA lncPARP1 contributes to progression of hepatocellular carcinoma through up-regulation of PARP1

Biosci Rep. 2018 Jun 21;38(3):BSR20180703. doi: 10.1042/BSR20180703. Print 2018 Jun 29.

Authors

Heqiang Qi¹, Yuyan Lu¹, Jie Lv¹, Huita Wu², Jing Lu¹, Changmao Zhang³, Sheng Zhang¹, Qing Bao¹, Xiuming Zhang⁴, Chengrong Xie⁵, Zhenyu Yin⁵

Affiliations

¹ Department of Hepatobiliary Surgery, Zhongshan Hospital, Xiamen University, Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, Xiamen, Fujian, P.R. China.
² Department of Oncology, Zhongshan Hospital, Xiamen University, Xiamen, Fujian, P.R. China.
³ Faculty of Clinical Medicine, Fujian Medical University, Fuzhou, Fujian, P.R. China.
⁴ Department of Biomaterials, College of Materials, Xiamen University, Xiamen, Fujian, P.R. China.
⁵ Department of Hepatobiliary Surgery, Zhongshan Hospital, Xiamen University, Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, Xiamen, Fujian, P.R. China eason_xiecr@126.com yinzy@xmu.edu.cn.

Abstract

Hepatocellular carcinoma (HCC) accounts for a large proportion of cancer-associated mortality worldwide. The functional impact of long noncoding RNAs (lncRNAs) in human cancer is not fully understood. Here, we identified a novel oncogenic lncRNA termed as lncPARP1, which was significantly up-regulated in HCC. Increase in lncPARP1 expression was associated with age, α-fetoprotein (AFP) levels, tumor size, recurrence, and poor prognosis of HCC patients. Loss-of-function approaches showed that knockdown of lncPARP1 inhibited proliferation, migration, and invasion, while induced apoptosis in HCC cells. Moreover, mechanistic investigation demonstrated that PARP1 was an underlying target of lncPARP1 in HCC. In summary, we provide the first evidence that lncPARP1 exerts an oncogene to promote HCC development and progression, at least in part, by affecting poly (ADP-ribose) (PAR) polymerase 1 (PARP1) expression.

Keywords: PARP1; growth; invasion; prognosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Animals
Carcinogenesis / genetics*
Carcinogenesis / metabolism
Carcinogenesis / pathology
Carcinoma, Hepatocellular / genetics*
Carcinoma, Hepatocellular / metabolism
Carcinoma, Hepatocellular / mortality
Carcinoma, Hepatocellular / pathology
Cell Line, Tumor
Cell Movement
Cell Proliferation
Disease Progression
Female
Gene Expression Regulation, Neoplastic*
Hep G2 Cells
Heterografts
Humans
Liver Neoplasms / genetics*
Liver Neoplasms / metabolism
Liver Neoplasms / mortality
Liver Neoplasms / pathology
Male
Mice, Nude
Middle Aged
Poly (ADP-Ribose) Polymerase-1 / genetics*
Poly (ADP-Ribose) Polymerase-1 / metabolism
RNA, Long Noncoding / antagonists & inhibitors
RNA, Long Noncoding / genetics*
RNA, Long Noncoding / metabolism
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism
Signal Transduction
Survival Analysis

Substances

RNA, Long Noncoding
RNA, Small Interfering
PARP1 protein, human
Poly (ADP-Ribose) Polymerase-1