Liposomes with phase transition temperatures, Tm, near pathogenic site temperature are potential chemoprophylactic delivery vehicles. We prepared and characterized the thermal properties of liposomes composed of 1,2-dipalmitoyl- sn-glycero-3-phosphocholine (DPPC) and hydrogenated soy phosphatidylcholine (HSPC) incorporating β-ionone with Tm at 42 °C. Liposomes with β-ionone/lipid ratio (w/w) of 1:20 and 1:8 had the necessary stability and released most of the β-ionone. The molecular architecture surrounding Tm was studied by fluorescent probes, Raman spectroscopy, and differential scanning calorimeter (DSC). β-Ionone was found to be preferentially located in the deep regions of the lipid bilayer (toward the long chain alkyl of the lipid) at moderate loading. The results showed that β-ionone encapsulated liposomes have a superior release at higher loading amount. Increasing β-ionone leads to disorder in the liquid crystalline state and accelerates the release rate. These studies provide information on the membrane structural properties of β-ionone loaded liposomes that guide rational bioactive molecular delivery systems design for health products.
Keywords: Raman spectroscopy; Thermosensitive liposomes (TSLs); differential scanning calorimetry; microviscosity; β-ionone.