The simultaneous loading of catechin and quercetin on chitosan-based nanoparticles as effective antioxidant and antibacterial agent

Feng Li; Huimiao Jin; Jie Xiao; Xusheng Yin; Xinying Liu; Dapeng Li; Qingrong Huang

doi:10.1016/j.foodres.2018.05.038

The simultaneous loading of catechin and quercetin on chitosan-based nanoparticles as effective antioxidant and antibacterial agent

Food Res Int. 2018 Sep:111:351-360. doi: 10.1016/j.foodres.2018.05.038. Epub 2018 May 21.

Authors

Feng Li¹, Huimiao Jin¹, Jie Xiao², Xusheng Yin³, Xinying Liu³, Dapeng Li⁴, Qingrong Huang⁵

Affiliations

¹ Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China.
² Department of Food Science, Rutgers University, New Brunswick, NJ 08901, USA.
³ Center of Bee Industry on Seed-Breeding and Popularization in Shandong Province, Taian 271000, China.
⁴ Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China. Electronic address: dpli73@sdau.edu.cn.
⁵ Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China; Department of Food Science, Rutgers University, New Brunswick, NJ 08901, USA. Electronic address: qhuang@sebs.rutgers.edu.

PMID: 30007696
DOI: 10.1016/j.foodres.2018.05.038

Abstract

A novel chitosan-based nanoparticle (G-C-Q NPs), which simultaneously loaded catechin and quercetin, was synthesized through ionic gelation reaction between chitosan and sodium tripolyphosphate and subsequent modification by genipin. Chemical structure, size distribution, morphology, entrapment efficiency, in vitro release behavior, antioxidant and antibacterial effects of G-C-Q NPs were investigated. Results showed that G-C-Q NPs were dispersed as an ellipsoidal shape with an average diameter of 180.4 nm and a zeta potential of 31.79 ± 1.28 mV. Entrapment efficiency of catechin and quercetin reached 76.35 ± 1.37% and 52.23 ± 2.45%, respectively. In vitro release study showed that both catechin and quercetin had a sustainable release from the G-C-Q NPs. G-C-Q NPs had higher or comparable potency in scavenging DPPH, ABTS⁺, OH and O₂^- radicals and in suppressing growing of Escherichia coli, Bacillus subtilis Staphylococcus aureus as compared to native drugs (single or combination). These results contribute some basis to the design and fabrication of polymeric nanoparticle delivery systems for multiple bioactive compounds with beneficial properties.

Keywords: (+)-Catechin (PubChem CID: 9064); Antibacterial; Antioxidant; Catechin; Chitosan; Chitosan (PubChem CID: 71853); Genipin (PubChem CID: 442424); Nanoparticles; Quercetin; Quercetin (PubChem CID: 5280343); Sodium tripolyphosphate (PubChem CID: 24455).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Antioxidants / pharmacology*
Bacillus subtilis / drug effects
Catechin / pharmacology*
Chelating Agents / chemistry
Chitosan / chemistry*
Escherichia coli / drug effects
Iridoids / chemistry
Nanoparticles
Quercetin / pharmacology*
Staphylococcus aureus / drug effects

Substances

Anti-Bacterial Agents
Antioxidants
Chelating Agents
Iridoids
Catechin
Chitosan
Quercetin
genipin