Abstract
Every transcription cycle ends in termination when RNA polymerase dissociates from the DNA. Although conceptually simple, the mechanism has proven somewhat elusive in eukaryotic systems. Gene-editing and high resolution polymerase mapping now offer clarification of important steps preceding transcriptional termination by RNA polymerase II in human cells.
Keywords:
3’ end processing; RNA degradation; RNA polymerase II (Pol II); Xrn2; cleavage and polyadenylation; transcriptional termination.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cells, Cultured
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Cleavage And Polyadenylation Specificity Factor / genetics
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Cleavage And Polyadenylation Specificity Factor / metabolism
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Escherichia coli / genetics
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Exoribonucleases / genetics
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Exoribonucleases / metabolism*
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Humans
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Models, Genetic
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Polyadenylation
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Promoter Regions, Genetic
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RNA 3' Polyadenylation Signals
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RNA Polymerase II / genetics
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RNA Polymerase II / metabolism*
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RNA Stability*
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Transcription Termination, Genetic*
Substances
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Cleavage And Polyadenylation Specificity Factor
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RNA Polymerase II
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Exoribonucleases
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XRN2 protein, human