Non-enzymatic electrochemical hydrogen peroxide biosensor based on reduction graphene oxide-persimmon tannin‑platinum nanocomposite

Hydrogen peroxide (H₂O₂) is one of the most universal and essential ingredients in distinct biological tissues. Herein, a novel non-enzymatic sensor based on reduction graphene oxide-persimmon tannin‑platinum nanocomposite (RGO-PT-Pt) was exploited for H₂O₂ detection. RGO-PT-Pt nanocomposite was prepared by reduction procedure with ascorbic acid as reducing agent and characterized by Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), ultraviolet visible spectroscopy (UV-vis) and Fourier infrared spectroscopy (FT-IR). Taking advantage of high electro-catalytic efficiency of Pt nanoparticles, high electronic conductivity and large surface area of RGO, and significant adsorption ability of PT on metal ions and its prevention of agglomeration to promote RGO dispersion, RGO-PT-Pt nanocomposite revealed better catalytic ability towards H₂O₂ via a synergistic effect. Under the optimal conditions, the RGO-PT-Pt non-enzymatic biosensor exhibited outstanding electrocatalytic activity towards H₂O₂ reduction. The amperometric response demonstrated a linear relationship with H₂O₂ concentration from 1.0 to100 μM with the correlation coefficient of 0.9931. The limit of detection was 0.26 μM (S/N = 3) and the response time was 3 s. Furthermore, the fabricated sensor exhibited a practical applicability in the quantification of H₂O₂ in human serum samples with an excellent recovery rate. Due to excellent performance such as fast response time, low detection limit, high stability and selectivity, the RGO-PT-Pt non-enzymatic biosensor has potential application in clinical diagnostics.