Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix

Claire Yu; Xuanyi Ma; Wei Zhu; Pengrui Wang; Kathleen L Miller; Jacob Stupin; Anna Koroleva-Maharajh; Alexandria Hairabedian; Shaochen Chen

doi:10.1016/j.biomaterials.2018.12.009

Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix

Biomaterials. 2019 Feb:194:1-13. doi: 10.1016/j.biomaterials.2018.12.009. Epub 2018 Dec 10.

Authors

Claire Yu¹, Xuanyi Ma², Wei Zhu¹, Pengrui Wang³, Kathleen L Miller¹, Jacob Stupin⁴, Anna Koroleva-Maharajh⁴, Alexandria Hairabedian⁴, Shaochen Chen⁵

Affiliations

¹ Department of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
² Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
³ Materials Science and Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
⁴ Chemical Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
⁵ Department of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Materials Science and Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Chemical Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA. Electronic address: chen168@eng.ucsd.edu.

Abstract

Decellularized extracellular matrices (dECMs) have demonstrated excellent utility as bioscaffolds in recapitulating the complex biochemical microenvironment, however, their use as bioinks in 3D bioprinting to generate functional biomimetic tissues has been limited by their printability and lack of tunable physical properties. Here, we describe a method to produce photocrosslinkable tissue-specific dECM bioinks for fabricating patient-specific tissues with high control over complex microarchitecture and mechanical properties using a digital light processing (DLP)-based scanningless and continuous 3D bioprinter. We demonstrated that tissue-matched dECM bioinks provided a conducive environment for maintaining high viability and maturation of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and hepatocytes. Microscale patterning also guided spontaneous cellular reorganization into predesigned striated heart and lobular liver structures through biophysical cues. Our methodology enables a light-based approach to rapidly bioprint dECM bioinks with accurate tissue-scale design to engineer physiologically-relevant functional human tissues for applications in biology, regenerative medicine, and diagnostics.

Keywords: 3D bioprinting; Biomimetic tissues; Decellularized extracellular matrix; Human induced-pluripotent stem cells; Tissue-specific bioinks.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bioprinting / methods*
Extracellular Matrix / chemistry*
Extracellular Matrix / ultrastructure
Hepatocytes / cytology
Humans
Induced Pluripotent Stem Cells / cytology*
Myocytes, Cardiac / cytology
Printing, Three-Dimensional
Swine
Tissue Engineering / methods*
Tissue Scaffolds / chemistry*

Abstract

Publication types

MeSH terms

Grants and funding