Inflammation plays an important role in the development of acute lung injury (ALI) in preterm infants. Despite the critical role of microRNA in inflammatory response, little is known about its function in ALI. In this study, we investigate the role of MicroRNA-24 (miR-24) in lipopolysaccharide (LPS) induced neonatal rats ALI and its potential mechanism. LPS was used to induce ALI neonatal animal model. miR-24 expression in the lung tissues of LPS-challenged neonatal rats was detected by qPCR. Proinflammatory factors, including tumor necrosis factor-alpha (TNF-α), IL-1β, IL-18 in the bronchoalveolar lavage fluid and lung tissues of LPS-challenged neonatal rats were measured by qRT-PCR and western blot, respectively. The mRNA levels of surfactant protein A (SP-A) and D (SP-D) was measured by qRT-PCR. Direct binding of miR-24 and pyrin domain-containing 3(NLRP3) were determined by dual luciferase assay. The levels of NLRP3, apoptosis-associated speck-like protein containing a C‑terminal caspase recruitment domain (ASC) and caspase-1 protein expression were detected by immunohistochemistry (IHC) staining and western blot, respectively. Our data indicated that LPS-induced lung injury in neonatal rats and resulted in significant downregulated of miR-24 expression. Overexpression of miR-24 significantly reduced LPS-induced lung damage and decreased the release of proinflammatory cytokine TNF-α, IL-6, IL-1β and SP-A, SP-D expression induced by LPS. In addition, miR-24 inhibited the expression of NLRP3 by directly targeting to the CDS region of NLRP3 mRNA. Furthermore, miR-24 overexpression attenuated lung inflammation and deactivated the NLRP3/caspase-1/IL-1β pathway in LPS-challenged neonatal rats. These data show that miR-24 alleviated inflammatory responses in LPS-induced ALI via targeting NLRP3.
Keywords: Acute lung injury; Inflammatory; Lipopolysaccharide; NLRP3; miR-24.
Copyright © 2018. Published by Elsevier GmbH.