Nanoscale fluorescent metal-organic framework composites as a logic platform for potential diagnosis of asthma

Asthma is a common chronic disorder, and the decreased hydrogen sulfide (H₂S) production in the lung has been considered as an early detection biomarker for asthma. However, the detection of H₂S in biological systems remains a challenge; because it requires the designed sensors to have the following features: nanoscale size, good biocompatibility, real-time detection, high selectivity/sensitivity, and good water stability. Here, we propose the potential of using nanoscale fluorescent metal-organic framework (MOF) composites Eu³⁺/Ag⁺@UiO-66-(COOH)₂ (hereafter denoted as EAUC) as a logic platform for tentative diagnosis of asthma by detecting the biomarker H₂S. This INHIBIT logic gate based on Eu³⁺@UiO-66-(COOH)₂ (EUC) can be produced by choosing Ag⁺ and H₂S as inputs and by monitoring the fluorescent signal (I₆₁₅) as an output. Our fluorescent studies indicate that the EAUC exhibits excellent selectivity, extreme sensitivity (limit of detection: 23.53 μM), and real-time in situ detection of H₂S. Further, MTT analysis in PC12 cells shows that the EAUC possesses low cytotoxicity and favourable biocompatibility that are suitable for the detection of biomarker H₂S in vivo, as demonstrated by the successful detection of spiked H₂S in the diluted serum samples. This work represents the possibility of using MOF-based logic platform for tentative diagnosis of asthma in clinical medicine.