Bioaugmentation-assisted phytoremediation of lead and salinity co-contaminated soil by Suaeda salsa and Trichoderma asperellum

The combined application of plant Suaeda salsa and indigenous fungus Trichoderma asperellum on the treatment of a lead (Pb) and salinity (Na⁺ and Ca²⁺) co-contaminated soil was investigated by a flowerpot experiment. As demonstrated by plant growth and selected antioxidant parameters, S. salsa was able to tolerate and grow in the co-contaminated soil, especially bioaugmented with T. asperellum, which promoted plant growth (9-23% and 5-13% increases for plant height and fresh weight, respectively) and appeared to alleviate plant oxidative damage (7-85% and 7-49% decreases for plant malondialdehyde and peroxidase levels, respectively). The SDS-PAGE fingerprints indicated that the total protein contents of S. salsa were affected under Pb and salinity stresses. The interactions of Na⁺ and Ca²⁺ ions on the phytotoxicity of Pb remained hormesis phenomenon that low-dose alleviation and high-dose enhancement. The analysis of phytoextraction parameters and bioavailability demonstrated that Pb was mainly concentrated in plant roots and poorly translocated, indicating the phytostabilization served as a major repair pathway. On the contrary, the Na⁺ and Ca²⁺ ions were concentrated in plant by the following order: shoot > root. Moreover, bioaugmentation of planted soil with T. asperellum generally led to the 9-42%, 13-58%, and 19-30% decreases of plant Pb, Na⁺, and Ca²⁺ concentrations and translocations, respectively, as well as a 6-21% decrease of soil Pb bioavailability. This study provided a bioaugmentation-assisted phytoremediation technique to make up the deficiencies of the long-term remediation for heavy metals and salinity.