Galleria mellonella as an experimental model to study human oral pathogens

Rodnei Dennis Rossoni; Felipe de Camargo Ribeiro; Hanna Flávia Santana Dos Santos; Jéssica Diane Dos Santos; Nicássia de Sousa Oliveira; Marignês Theotonio Dos Santos Dutra; Simone Aparecida Biazzi de Lapena; Juliana Campos Junqueira

doi:10.1016/j.archoralbio.2019.03.002

Galleria mellonella as an experimental model to study human oral pathogens

Arch Oral Biol. 2019 May:101:13-22. doi: 10.1016/j.archoralbio.2019.03.002. Epub 2019 Mar 5.

Authors

Rodnei Dennis Rossoni¹, Felipe de Camargo Ribeiro², Hanna Flávia Santana Dos Santos³, Jéssica Diane Dos Santos⁴, Nicássia de Sousa Oliveira⁵, Marignês Theotonio Dos Santos Dutra⁶, Simone Aparecida Biazzi de Lapena⁷, Juliana Campos Junqueira⁸

Affiliations

¹ Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil. Electronic address: dennisrossoni@hotmail.com.
² Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil. Electronic address: felipe_c_ribeiro@hotmail.com.
³ Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil. Electronic address: hannasantana.f@gmail.com.
⁴ Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil. Electronic address: jessicadiane.santos@yahoo.com.br.
⁵ Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil. Electronic address: nykassya@hotmail.com.
⁶ Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil. Electronic address: marignes.theotonio@unesp.br.
⁷ Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil. Electronic address: simone_lapena@yahoo.com.br.
⁸ Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil. Electronic address: juliana@ict.unesp.br.

PMID: 30856377
DOI: 10.1016/j.archoralbio.2019.03.002

Abstract

The oral cavity is home to a microbial community of more than 800 species. This important microbiome is formed by commensal and opportunistic bacteria, fungi and viruses. Several distinct habitats within the mouth support heterogeneous microbial communities that constitute an important link between oral and general health. The use of animal models for in vivo studies in microbial pathogenicity is well established in the scientific community. Galleria mellonella as a model host has increased in use significantly in the last few years. This invertebrate model provides studies on a large scale, serving as screens for studies on vertebrate animals, such as mice and rats. In this review, different studies of microbial genera of dental importance (Enterococcus, Candida, Lactobacillus, Porphyromonas and Streptococcus) are discussed, highlighting the use of G. mellonella as a suitable model for studying pathogenesis, efficacy of antimicrobial compounds, and immune responses.

Keywords: Experimental model; Galleria mellonella; Oral pathogens; Pathogenesis.

Publication types

Review

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Bacterial Infections*
Disease Models, Animal*
Humans
Larva
Models, Theoretical
Moths*
Mouth Diseases / microbiology*
Mycoses*
Virulence

Substances

Anti-Bacterial Agents