Aflatoxins screening in non-dairy beverages by Mn-doped ZnS quantum dots - Molecularly imprinted polymer fluorescent probe

Anis Chmangui; Mohamed Ridha Driss; Soufiane Touil; Pilar Bermejo-Barrera; Sondes Bouabdallah; Antonio Moreda-Piñeiro

doi:10.1016/j.talanta.2019.02.057

Aflatoxins screening in non-dairy beverages by Mn-doped ZnS quantum dots - Molecularly imprinted polymer fluorescent probe

Talanta. 2019 Jul 1:199:65-71. doi: 10.1016/j.talanta.2019.02.057. Epub 2019 Feb 14.

Authors

Anis Chmangui¹, Mohamed Ridha Driss², Soufiane Touil², Pilar Bermejo-Barrera³, Sondes Bouabdallah², Antonio Moreda-Piñeiro⁴

Affiliations

¹ Laboratory of Hetero-Organic Compounds and Nanostructured Materials (LR18ES11), Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna, Tunisia; Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT), Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s/n., 15782 Santiago de Compostela, Spain.
² Laboratory of Hetero-Organic Compounds and Nanostructured Materials (LR18ES11), Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna, Tunisia.
³ Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT), Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s/n., 15782 Santiago de Compostela, Spain.
⁴ Trace Element, Spectroscopy and Speciation Group (GETEE), Strategic Grouping in Materials (AEMAT), Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, Universidade de Santiago de Compostela, Avenida das Ciencias, s/n., 15782 Santiago de Compostela, Spain. Electronic address: antonio.moreda@usc.es.

PMID: 30952310
DOI: 10.1016/j.talanta.2019.02.057

Abstract

Synthesized molecularly imprinted polymer (MIP) materials have been anchored on the surface of PEG-Mn-doped ZnS quantum dots (QDs) to develop a fluorescence probe for aflatoxins (AFs) recognition/determination in non-dairy beverages. MIP synthesis used 5,7-dimethoxycoumarin (DMC) as a dummy template molecule, and methacrylic acid (MAA) as a functional monomer. Under optimized conditions (1.25 mL of 5 mg L^-1 MIP-PEG-ZnS QDs solution, pH 5.0, and 12 min delay time before scanning), the prepared MIP-QDs composite was found to offer high affinity and selectivity for AFs (AFB1, AFB2, AFG1 and AFG2). A fast fluorimetric screening method (total AFs assessment) was therefore feasible. The limits of detection (LOD) and quantification (LOQ) were 0.016 and 0.053 mg L^-1, respectively. Analytical recoveries (inter- and intra-day assays) were from 99 ± 4% to 107 ± 5%, with RSD (intra-day assay) lower than 13, and RSD (inter-day) lower than 7%. The optimized method was applied for total AFs assessment in several non-dairy beverage samples.

Keywords: Aflatoxins; Fluorescence probe; Molecularly imprinted polymer; Non-dairy beverages; Quantum dots.

MeSH terms

Aflatoxins / analysis*
Beverages / analysis*
Fluorescent Dyes / chemistry*
Manganese / chemistry
Molecular Imprinting*
Polymers / chemistry*
Quantum Dots / chemistry*
Sulfides / chemistry
Zinc Compounds / chemistry

Substances

Aflatoxins
Fluorescent Dyes
Polymers
Sulfides
Zinc Compounds
Manganese
zinc sulfide