This study was aimed to determine the relationship of flavonoid structures to their affinity for an important efflux transporter, multidrug-resistant associated protein 2 (MRP2). The cellular uptake (CU) of 35 flavonoids was investigated in MRP2 overexpression MDCK/MRP2 cells. Resulting data identified 8 flavonoids as MRP2 substrates based on their high CUMK with MK-571 in MDCK/MRP2 cells. Also, three substrates showed better CUMD in MDCK cells than did CUMRP in MDCK/MRP2 cells. Docking analyses showed a good correlation (R = 0.926, p = 0.003) between efflux-fold of flavonoid substrates and their docking S_scoring with the MRP2 model, indicating consistency between in silico and in vitro approaches. A structure affinity relationship (SAR) study indicated that 3-OH, 5-OH, 6-OH, 3'-OH, and 4'-OCH3 substituents were favourable while, 8-OCH3, 2'-OH, 3'-OCH3, 4'-OH and 5'-OH were unfavourable for flavonoid affinity to MRP2. Our study provides valuable information for dietary application of flavonoids with specific structures for high absorption.
Keywords: Apigenin (PubChem CID: 5280443); Baicalein (PubChem CID: 5281605); Cellular uptake; Chrysin (PubChem CID: 5281607); Flavone (PubChem CID: 10680); Flavonoids; Galangin (PubChem CID: 5281616); Isovitexin (PubChem CID: 162350); Luteolin (PubChem CID: 5280445); MRP2; Schaftoside (PubChem CID: 442658); Structure affinity relationship; Substrates; Tangeretin (PubChem CID: 68077); Vitexin (PubChem CID: 5280441); Wogonin (PubChem CID: 5281703).
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