The objective of this study was to evaluate the effects of feeding purple corn (Zea mays L.) silage on productivity and blood superoxide dismutase concentration in lactating cows. We hypothesized that feeding purple corn silage (AX-152; Nagano Animal Industry Experiment Station, Nagano, Japan, and Takii and Co. Ltd., Tokyo, Japan), which is high in anthocyanin content, would increase milk production and blood concentration of superoxide dismutase. We assigned 16 Holstein cows (8 primiparous and 8 multiparous) in mid lactation to 1 of 2 treatments in a randomized block design, with efforts to balance parity, body weight, and days in milk between treatments. Experimental diets contained either purple corn silage [PCS; 31.2% dry matter (DM), 8.4% crude protein, 40.2% neutral detergent fiber, and 26.6% starch] or conventional corn silage (CONT; 30.5% dry matter, 8.7% crude protein, 42.1% neutral detergent fiber, and 26.5% starch) at approximately 32% of diet DM. Both PCS and CONT were ensiled for 5 mo before the study. Treatment diets were fed as total mixed rations ad libitum for 12 wk from February 1 to April 25, 2016. Cows fed the PCS had increased milk yield (31.7 vs. 29.2 kg/d) and blood superoxide dismutase concentrations (9,333 vs. 8,467 U/mL) compared with those fed CONT. However, anthocyanin concentration in the PCS decreased over the 12-wk experiment: 70 mg/kg of DM for the first 4 wk, 20 mg/kg of DM for the second 4 wk, and undetectable for the last 4 wk. We did not detect anthocyanins in the CONT group at any time point. Feeding PCS may increase antioxidant capacity and milk production in dairy cows, but anthocyanin in PCS may be degraded during storage.
Keywords: anthocyanin; antioxidant; oxidative stress.
The Authors. Published by FASS Inc. and Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).