Emergence of Klebsiella pneumoniae ST11 co-producing NDM-1 and OXA-48 carbapenemases in Greece

Efthymia Protonotariou; Georgios Meletis; Fani Chatzopoulou; Andigoni Malousi; Dimitrios Chatzidimitriou; Lemonia Skoura

doi:10.1016/j.jgar.2019.08.020

Emergence of Klebsiella pneumoniae ST11 co-producing NDM-1 and OXA-48 carbapenemases in Greece

J Glob Antimicrob Resist. 2019 Dec:19:81-82. doi: 10.1016/j.jgar.2019.08.020. Epub 2019 Sep 4.

Authors

Efthymia Protonotariou¹, Georgios Meletis², Fani Chatzopoulou³, Andigoni Malousi⁴, Dimitrios Chatzidimitriou³, Lemonia Skoura¹

Affiliations

¹ Department of Microbiology, AHEPA University Hospital, S. Kiriakidi Str. 1, 54636 Thessaloniki, Greece.
² Department of Microbiology, AHEPA University Hospital, S. Kiriakidi Str. 1, 54636 Thessaloniki, Greece. Electronic address: meletisg@hotmail.com.
³ Laboratory of Microbiology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
⁴ Laboratory of Biological Chemistry, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.

PMID: 31493526
DOI: 10.1016/j.jgar.2019.08.020

Abstract

Objectives: Klebsiella pneumoniae is a well-known pathogen frequently implicated in serious life-threatening nosocomial infections. Here we present a K. pneumoniae isolate (AHEPA1046) co-harbouring bla_NDM-1 and bla_OXA-48 isolated from a blood sample of an inpatient in Thessaloniki, Greece.

Methods: Whole-genome sequencing (WGS) was performed using an Illumina MiniSeq Sequencing System. Multilocus sequence typing (MLST) was performed using a BLAST-based approach, and antimicrobial resistance genes and plasmid replicons were identified by ResFinder and PlasmidFinder, respectively. The Rapid Annotation using Subsystem Technology (RAST) v.2.0 server was used for genome annotation.

Results: WGS analysis revealed the complete resistome of K. pneumoniae AHEPA1046. The strain harboured bla_NDM-1 and bla_OXA-48 together with 16 additional antimicrobial resistance genes and was resistant to carbapenems, aminoglycosides, quinolones, macrolides, tetracyclines, trimethoprim, fosfomycin and phenicols. Moreover, it was classified as ST11.

Conclusion: This is the first report of a K. pneumoniae clinical isolate from Greece co-producing NDM-1 and OXA-48 carbapenemases and is one of a few reported worldwide.

Keywords: Greece; Klebsiella pneumoniae; NDM-1; OXA-48; ST11; Whole-genome sequencing.

MeSH terms

Adult
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / blood
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism*
Base Composition
Base Sequence
Drug Resistance, Multiple, Bacterial / genetics*
Genes, Bacterial / genetics
Greece
Humans
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / metabolism*
Male
Multilocus Sequence Typing
Plasmids
Whole Genome Sequencing
beta-Lactamases / blood
beta-Lactamases / genetics*
beta-Lactamases / metabolism*

Substances

Anti-Bacterial Agents
Bacterial Proteins
beta-Lactamases
beta-lactamase NDM-1
carbapenemase
oxacillinase