Genomic characterization of a novel virulent phage infecting the Aeromonas hydrophila isolated from rainbow trout (Oncorhynchus mykiss)

Yongsheng Cao; Shaowu Li; Di Wang; Jingzhuang Zhao; Liming Xu; Hongbai Liu; Tongyan Lu; Zhenbo Mou

doi:10.1016/j.virusres.2019.197764

Genomic characterization of a novel virulent phage infecting the Aeromonas hydrophila isolated from rainbow trout (Oncorhynchus mykiss)

Virus Res. 2019 Nov:273:197764. doi: 10.1016/j.virusres.2019.197764. Epub 2019 Sep 21.

Authors

Yongsheng Cao¹, Shaowu Li², Di Wang³, Jingzhuang Zhao⁴, Liming Xu⁵, Hongbai Liu⁶, Tongyan Lu⁷, Zhenbo Mou⁸

Affiliations

¹ Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Songfa Street No. 43, Daoli District, Harbin, 150070, China; Institute of Fisheries Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, 130 Jinzhu West Road, Lhasa, 850002, Tibet, China. Electronic address: caoyongsheng@hrfri.ac.cn.
² Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Songfa Street No. 43, Daoli District, Harbin, 150070, China. Electronic address: lishaowu@hrfri.ac.cn.
³ Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Songfa Street No. 43, Daoli District, Harbin, 150070, China. Electronic address: wangdi@hrfri.ac.cn.
⁴ Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Songfa Street No. 43, Daoli District, Harbin, 150070, China. Electronic address: zhaojingzhuang@hrfri.ac.cn.
⁵ Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Songfa Street No. 43, Daoli District, Harbin, 150070, China. Electronic address: lmxu0917@163.com.
⁶ Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Songfa Street No. 43, Daoli District, Harbin, 150070, China. Electronic address: liuhongbai@sina.com.
⁷ Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Songfa Street No. 43, Daoli District, Harbin, 150070, China. Electronic address: lutongyan@hrfri.ac.cn.
⁸ Institute of Fisheries Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, 130 Jinzhu West Road, Lhasa, 850002, Tibet, China. Electronic address: mouzhenbo@163.com.

PMID: 31550486
DOI: 10.1016/j.virusres.2019.197764

Abstract

The virulent bacteriophage MJG that specifically infects Aeromonas hydrophila was isolated from a water sample from a river in Harbin, China. The genome of phage MJG was a double-stranded linear DNA with 45,057 bp, possessing 50.11% GC content. No virulence or resistance genes were found in the phage genome. Morphological observation, genomic characterization, and phylogenetic analysis indicated that MJG was closely related to phages belonging to the genus Sp6virus in the Podoviridae family. This phage is a novel member within Sp6virus that could infect and lyse A. hydrophila. This study could serve as a genomic reference of A. hydrophila phages and provide a potential agent for phage therapy.

Keywords: Aeromonas hydrophila; Bacteriophage; Genome analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aeromonas hydrophila / virology*
Animals
Bacteriophages / classification*
Bacteriophages / isolation & purification
Bacteriophages / pathogenicity*
Base Composition
China
Genome, Viral*
Genomics
Host Specificity
Oncorhynchus mykiss / microbiology*
Phylogeny
Podoviridae / genetics
Rivers
Water Microbiology