Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal mouse Macrophages by Flow Cytometry

Katharina Richard; Stefanie N Vogel; Darren J Perkins

doi:10.21769/BioProtoc.3369

Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal mouse Macrophages by Flow Cytometry

Bio Protoc. 2019 Sep 20;9(18):e3369. doi: 10.21769/BioProtoc.3369.

Authors

Katharina Richard¹, Stefanie N Vogel¹, Darren J Perkins¹

Affiliation

¹ Department of Microbiology and Immunology, University of Maryland Baltimore, Baltimore, USA.

Abstract

Internalization of cell surface Toll Like Receptor 4 (TLR4) is a convenient read-out to measure LPS dependent activation of the TRIF adaptor pathway. We here provide a protocol to quantify the LPS dependent internalization of TLR4 using thioglycollate-elicited peritoneal macrophages by flow cytometry.

Keywords: CD14; Endocytosis; LPS; TLR4; TRIF; peritoneal macrophages.

Abstract

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