Construction of a hydrocarbon-degrading consortium and characterization of two new lipopeptides biosurfactants

Apparent solubility and bioavailability of hydrophobic compounds are the major problems in the bioremediation process, which could be overcome by the bacteria capable of biosurfactant production and concurrent hydrocarbon degradation. In this work, we constructed an artificial bacterial consortium containing Lysinibacillus, Paenibacillus, Gordonia and Cupriavidus spp. from glyceryl tributyrate enriched bacteria collected from the non-contaminated site. The consortium was capable of using common raw materials (olive oil, paraffin oil, and glycerol) and polyaromatic hydrocarbons pollutants (naphthalene and anthracene) as the sole carbon source with simultaneous biosurfactant production. Two new lipopeptide isoforms, containing heptapeptide and lipid moieties, were structurally elucidated by LC-MS/MS, FTIR, NMR and molecular networking analysis. Our findings indicate that hydrocarbons degradation and biosurfactant production is an intrinsic property of non-contaminated soil community. Interestingly, we observed the hyper chemotactic activity of Lysinibacillus strains towards glyceryl tributyrate, which has not been reported before. The study may deepen our understanding of microbial strains and consortium with the potential to be used for bioremediation of hydrocarbons contaminated environments.