Abstract
Single-particle cryo-electron microscopy has become an indispensable technique in structural biology. In particular when studying membrane proteins, it allows the use of membrane-mimicking tools, which can be crucial for a comprehensive understanding of the structure-function relationship of the protein in its native environment. In this chapter we focus on the application of nanodiscs and use our recent studies on the TMEM16 family as an example.
Keywords:
Cryo-EM; Membrane proteins; Nanodiscs; Single-particle; Structural biology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anoctamins / chemistry
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Anoctamins / metabolism
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Cryoelectron Microscopy / methods*
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Data Collection / methods
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Fungal Proteins / chemistry
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Fungal Proteins / metabolism
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Fusarium
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Humans
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Image Processing, Computer-Assisted / methods
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Lipid Bilayers / chemistry*
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Lipid Bilayers / metabolism
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Membrane Proteins / chemistry*
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Membrane Proteins / metabolism
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Models, Molecular
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Nanostructures / chemistry
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Protein Conformation
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Single Molecule Imaging / methods*
Substances
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Anoctamins
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Fungal Proteins
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Lipid Bilayers
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Membrane Proteins