Screening lambdagt recombinant clones by hybridization to single plaques in situ

Science. 1977 Apr 8;196(4286):180-2. doi: 10.1126/science.322279.

Abstract

A rapid, direct method for screening single plaques of Agt recombinant phage is described. The method allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Coliphages / analysis*
  • DNA, Recombinant / analysis*
  • DNA, Viral / analysis*
  • Escherichia coli
  • Nucleic Acid Hybridization*

Substances

  • DNA, Recombinant
  • DNA, Viral