Effect of salt stress on in vitro organogenesis from nodal explant of Limnophila aromatica (Lamk.) Merr. and Bacopa monnieri (L.) Wettst. and their physio-morphological and biochemical responses

Salinity is one of the most severe abiotic stress factors that limit crop productivity by affecting the growth of plants. Therefore, it is significant to know the responses of plants against salt stress. In this study, the callus formation capabilities of nodal explants of Limnophila aromatica (Lamk.) Merr. and Bacopa monnieri (L.) Wettst. incubated under different NaCl concentrations (0-100 mM) in in vitro culture conditions were investigated and also the effect of NaCl on the release of regenerated shoots from these calluses was examined. Furthermore, the plants under NaCI stress were evaluated physiologically and biochemically. Callus formation percentages and callus intensities from the nodal explants decreased with increasing NaCl concentrations. In addition, yellowing, browning and even deaths were observed in calluses under salt toxicity. The callus was taken into the subculture, and the increased NaCl concentration in both plant species adversely affected the regeneration ability of the shoots. The number of shoots per callus for L. aromatica and B. monnieri was 6.72-17.49 and 7.42-15.38, respectively. The length of shoots in L. aromatica was between 0.95 and 1.65 cm, and in B. monnieri between 1.17 and 1.81 cm. The lowest number of shoots per callus and the shoot lengths were found in medium containing 100 mM NaCl. Moreover, photosynthetic pigmentation, lipid peroxidation, protein content, and proline content was damaged with increased salinity compared to the control group. This comprehensive study in tissue culture conditions can a be potential contributor to the literature and can help other studies to be carried out in the future.